CRISPR/Cas9 Genome Editing Service of Leishmania

CRISPR/Cas9 Genome Editing Service of Leishmania

CRISPR/Cas9 Genome Editing for Leishmania

Leishmania refers to a broad family of trypanosomatid worms of the genus Leishmania that cause leishmaniasis. The main hosts of Leishmania are vertebrates, and common infections include cervids, rodents, canids, and humans. There are two life histories: one is to parasitize and reproduce in macrophages and other cells of the reticuloendothelial system of the spleen, liver, bone marrow, lymph nodes, and mucous membranes of vertebrates; When the cells rupture, the escaped worms can reinvade new macrophages.

With its simple design, flexible application, easy operation and high efficiency, the CRISPR/Cas9 system has played an important role in editing the genes of Leishmania, improving the efficiency of gene editing and gaining initial applications in the study of its gene function, drug resistance, drug targets and vaccines. As we optimize and upgrade the system, the CRISPR/Ca9 system will better help researchers to understand Leishmania at the genetic level, thus effectively combating leishmaniasis and promoting human health.

Ace Therapeutics, as a genome editing service provider, is focused on leveraging CRISPR/Cas9 technology and high-throughput screening to advance basic research and the pharmaceutical industry. We can provide CRISPR/Cas9 gene editing services for Leishmania coccinea to lay the foundation for in-depth elucidation of mechanistic studies.

CRISPR/Cas9 Genome Editing Service Options for Leishmaniasis

We have a technical team with rich experience in CRISPR gene editing, and we can now realize one-stop service including gRNA design, CRISPR/Cas9 vector construction, transfection, and identification. After the service is completed, we will deliver you CRISPR/Cas9 gene editing products and detailed identification reports. If you want to know more, you are welcome to consult us.
Leishmania's CRISPR /Cas9 cell line building technology services

  • CRISPR/Cas9 knockout cell lines
  • CRISPR/Cas9 targeted gene knock-in cell lines
  • CRISPR/Cas9 reporter knock-in cell lines
  • CRISPR/Cas9 targeted mutation cell lines
  • CRISPR /Cas9 miRNA/ IncRNA knockout cell lines
  • CRISPR/Cas9 multiple knockout cell lines

CRISPR/Cas9 service content

  • CRISPR target site selection
  • gRNA design
  • CRISPR vector construction
  • Oligonucleotide donor and resistance selection donor design
  • Cell transfection of Leishmania protozoa
  • Screening and isolation of CRISPR-edited mutants.

Customers need to provide

  • Leishmania strain
  • Target gene related information
    1) Knockout: ID of knockout gene, lethality of pre-knockout gene
    2) Knock-in: sequence of knock-in DNA, knock-in site
    3) Point mutation: point mutation location and base change

Service Details

Delivery content: experiment report, gene editing cell lines, Leishmania or Leishmania animal models that edited by CRISPR/Cas9 technology
Test fee: please get it through online inquiry.

Service Flow

  1. Provide a preliminary project plan according to the customer's needs.
  2. The company's technical team comprehensively assesses the feasibility of the project, works with the customer to determine the exact experimental project execution plan and gives the exact experimental price.
  3. The customer signs the service agreement and prepays part of the fee, we do the initial preparation of the experiment and project prearrangement.
  4. We formally start the experimental process, fully launch the experiment, and ensure the effective implementation of the project.
  5. We communicate with the customer and give feedback on the latest progress of the experiment, and keep abreast of the latest developments of the experiment.
  6. The client pays the remaining balance, we deliver the experimental results and provide after-sales service.

Our Advantages

  • We have a strong technical support team and can provide free professional technical support for our customers.
  • Authoritative detection methods and standardized service process.
  • Short experimental cycle time and low price.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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