As endophytes live in the special environment of the plant body for a long time and co-evolve with the host, they form a mutually beneficial symbiotic relationship during the evolutionary process. On the one hand, plants provide photosynthetic products and minerals for endophytes to grow, and on the other hand, endophytes play an important role in the growth, development, and phylogeny of the host. Of course, there are many unknown things about endophytic bacteria, such as how the metabolites of endophytic bacteria promote the growth mechanism of crop yield and active ingredients of medicinal plants, and the use of endophytic bacteria in biofertilizers has the effect of improving plant nutrition, which has great research prospects in several fields.
Ace Therapeutics provides a one-stop shop for the multi-omics of plant endophytes, including but not limited to genomics, transcriptomics, proteomics, metabolomics, and spatial omics. This helps you to understand the basic characteristics of endophytic bacteria and grasp their variation patterns, promotes the exploration of the mechanism of endophytic bacteria-plant interactions and the analysis of the symbiosis mechanism between endophytic bacteria and hosts in a fully functional body, and provides new strategies for plant nutrition and disease resistance breeding from the endophytic perspective. From sample extraction, library construction, sequencing/assay to data analysis, each step is scientifically and carefully designed to ensure high-quality results.
There are also a wide variety of plant endophytes, including three main groups: endophytic bacteria, endophytic fungi, and endophytic actinomycetes.
The plant endophytic bacteria found in various crops and cash crops are mainly Pseudomonas, Enterobacter, Bacillus, Agrobacterium, Klebsiella, Pantoea (Methylobacterium), etc.
Endophytic fungi are mainly studied in medicinal plants, lambsquarters, and tropical trees. The endophytic fungi found are mainly comet and their asexual types, including Pyrenomyetes, Discomyetes, and Loculoascomyetes.
Plant endophytic actinomycetes are mainly studied in mangroves, tropical perennial trees, and some medicinal crops, Streptomyces, Streptoverticillum, Antinoplanes, Nocardia, and Micromonospora.
1. Metabolomics and proteomics
We provide proteomic studies of plant endophytes for our customers worldwide. We have iTRAQ/TMT labeling quantification and label-free quantification, as well as DIA quantification technologies for your flexible choice. We also provide metabolomics services for plants/plant endophytes. We can help you to analyze how endophytes compete with pathogens for space and nutrients, how endophytes secrete metabolites such as antibiotics/toxins, how these metabolites induce systemic resistance (ISR) in plants, how endophytic fungi perform nitrogen fixation, phosphate solubilization, phytohormone production, change root morphology, regulate infiltration, increase iron phosphate carrier production, enhance solubilization activity, and regulate stomata, etc.
2. Metagenomics
The number and species of endophytic bacteria present in various plant bodies are closely related to the plant species, living environment, growth stage, nutrient supply, and genotype of both. Plant endophyte metagenomics can help you to resolve the above questions, and also provide new strategies for research into breeding for plant microbial origins, plant nutrition, and disease resistance from an endophyte perspective. We provide metagenomics services for plant endophytes, including species classification, phylogeny, gene function annotation, and metabolic network analysis. We have the advantages of short cycle time, high throughput, and complete information.
Note: In addition, there are other services available for you to choose from, please consult us for details.
The application of plant endophytes to plants to produce antimicrobial/anti-insecticides enhances plant resistance and provides biological control effects, which reduces the pollution of the environment by chemical pesticides and ensures the yield quality of plants.
Plant endophytic bacteria metabolites have antitumor, antibacterial, and antiviral effects. The development of new drugs using secondary metabolites of plant endophytes will be one of the future research directions in medicine.
Due to the adaptability of endophytic bacteria in plants, some researchers have constructed endophytic disease-preventing or insecticidal engineering bacteria in plants using endophytic bacteria as receptors and then introduced them into plants to make plants play the same or similar role as genetically modified disease-preventing and insecticidal plants, thus achieving biological control.
In addition, plant endophytic bacteria have been found to have the function of degrading environmental pollutants.